Aptamer-based electrochemical biosensor by using Au-Pt nanoparticles, carbon nanotubes and acriflavine platform
作者: Zeinab Sahraei BeiranvandAmir Reza AbbasiSara DehdashtianZiba KarimiAzadeh Azadbakht
作者单位: 1Department of Chemistry, Khorramabad Branch, Islamic Azad University, Khorramabad, Iran
2Department of Chemistry, Islamic Azad University, Omidieh Branch, Omidieh, Iran
刊名: Analytical Biochemistry, 2017, Vol.518 , pp.35-45
来源数据库: Elsevier Journal
DOI: 10.1016/j.ab.2016.10.001
关键词: AptasensorGold–platinum nanoparticlesBisphenol AAcriflavine
英文摘要: Abstract(#br)Herein, an ultrasensitive electrochemical aptasensor for quantitative detection of bisphenol A (BPA) was fabricated based on a novel signal amplification strategy. This aptasensor was developed by electrodeposition of gold-platinum nanoparticles (Au-PtNPs) on glassy carbon (GC) electrode modified with acid-oxidized carbon nanotubes (CNTs-COOH). In this protocol, acriflavine (ACF) was covalently immobilized at the surface of glassy carbon electrode modified with Au-PtNPs/CNTs-COOH nanocomposite. Attachment of BPA-aptamer at the surface of modified electrode was performed through the formation of phosphoramidate bonds between the amino group of ACF and phosphate group of the aptamer at 5′end. By interaction of BPA with the aptamer, the conformational of aptamer was changed...
全文获取路径: Elsevier  (合作)
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影响因子:2.582 (2012)

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关键词翻译
关键词翻译
  • acriflavine 吖啶黄素
  • platform 台地
  • based 基于
  • carbon 
  • biosensor 生物传感器生物感测器