Simultaneous determination of albumin and low-molecular-mass thiols in plasma by HPLC with UV detection
作者: Kamila BorowczykMonika Wyszczelska-RokielPaweł KubalczykRafał Głowacki
作者单位: 1University of Łódź, Faculty of Chemistry, Department of Environmental Chemistry, 163 Pomorska Str., 90-236 Łódź, Poland
刊名: Journal of Chromatography B, 2015, Vol.981-982 , pp.57-64
来源数据库: Elsevier Journal
DOI: 10.1016/j.jchromb.2014.12.032
关键词: AlbuminEndogenic plasma thiolsHigh performance liquid chromatographyDerivatizationUltraviolet detection
原始语种摘要: Abstract(#br)In this paper, we describe a simple and robust HPLC based method for determination of total low- and high-molecular-mass thiols, protein S-linked thiols and reduced albumin in plasma. The method is based on derivatization of analytes with 2-chloro-1-methylquinolinium tetrafluoroborate, separation and quantification by reversed-phase liquid chromatography followed by UV detection. Disulfides were converted to their thiol counterparts by reductive cleavage with tris(2-carboxyethyl)phosphine. Linearity in detector response for total thiols was observed over the range of 1–40μmolL −1 for Hcy and glutathione (GSH), 5–100μmolL −1 for Cys–Gly, 20–300μmolL −1 for Cys and...
全文获取路径: Elsevier  (合作)
影响因子:2.487 (2012)