Clusters of biotin-responsive genes in human peripheral blood mononuclear cells
作者: Silke WiedmannRocio Rodriguez-MelendezDaniel Ortega-CuellarJanos Zempleni
作者单位: 1Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, 316 Ruth Leverton Hall, Lincoln, NE 68583-0806, USA
2Departments of Biochemistry and Animal Science, University of Nebraska at Lincoln, Lincoln, NE, USA
刊名: The Journal of Nutritional Biochemistry, 2004, Vol.15 (7), pp.433-439
来源数据库: Elsevier Journal
DOI: 10.1016/j.jnutbio.2004.02.005
关键词: BiotinDNA microarraysGene expressionHumanPeripheral blood mononuclear cells
原始语种摘要: Abstract(#br)Effects of biotin in cell signaling are mediated by transcription factors such as nuclear factor–κB (NF-κB) and Sp1/Sp3 as well as by posttranslational modifications of DNA-binding proteins. These signaling pathways play roles in the transcriptional regulation of numerous genes. Here we tested the hypothesis that biotin-dependent genes are not randomly distributed in the human genome but are arranged in clusters. Peripheral blood mononuclear cells were isolated from healthy adults before and after supplementation with 8.8 μmol/day biotin for 21 days. Cells were cultured ex vivo with concanavalin A for 3 hours to stimulate gene expression. Abundances of mRNA encoding ∼14,000 genes were quantified by both DNA microarray and reverse transcriptase–polymerase chain reaction. The...
全文获取路径: Elsevier  (合作)
影响因子:4.552 (2012)

  • responsive 承担责任
  • biotin 生物素
  • expression 表示
  • whereas 鉴于
  • transcriptase 转录酶
  • human 人的
  • numerous 许多的
  • genome 基因组
  • cells 麻风细胞
  • clustered 群生的