Reconstitution of the basal calcium transport in resealed human red blood cell ghosts
作者: Roman HudecBoris LakatošJozef OrlickýL’udovít Varečka
作者单位: 1Department of Biochemistry and Microbiology, Slovak University of Technology, 812 37-Bratislava, Slovak Republic
2Institute of Molecular Physiology and Genetics, Slovak Academy of Sciences, 833 34-Bratislava, Slovak Republic
刊名: Biochemical and Biophysical Research Communications, 2004, Vol.325 (4), pp.1172-1179
来源数据库: Elsevier Journal
DOI: 10.1016/j.bbrc.2004.10.167
关键词: Human red blood cellsResealed ghostsBasal Ca 2+ influxBand 3UncouplerPMA
英文摘要: Abstract(#br)The 45 Ca 2+ influx into right-side-out resealed ghosts (RG) prepared from human red blood cells (RBC) was measured. The 45 Ca 2+ equilibration occurred with t 1/2 =2.5min and the steady-state was reached after 17min with the level of 22±2μmol/L packed cells at 37°C. The rate of the influx was 97±17μmol/L packed cells h. The 45 Ca 2+ influx was saturated with [Ca 2+ ] 0 at 4mmol/L and was optimal at pH 6.5 and 30°C. Divalent cations (10 −4 –10 −6 mol/L), nifedipine (10 −5 –10 −4 ...
全文获取路径: Elsevier  (合作)
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关键词翻译
关键词翻译
  • blood 血液
  • transport 输运
  • basal 基部
  • cells 麻风细胞
  • influx 
  • calcium 
  • human 人的
  • PMA Physical Memory Address