Application of GC–MS technique for the determination of homocysteine thiolactone in human urine
作者: Monika WrońskaGrażyna ChwatkoKamila BorowczykJustyna PiechockaPaweł KubalczykRafał Głowacki
作者单位: 1University of Łódź, Faculty of Chemistry, Department of Environmental Chemistry, 163 Pomorska Str., 90-236 Łódź, Poland
刊名: Journal of Chromatography B, 2018
来源数据库: Elsevier Journal
DOI: 10.1016/j.jchromb.2018.09.009
关键词: GC–MSHomocysteine thiolactoneHuman urineIsobutyl chloroformateLiquid-liquid extraction
原始语种摘要: Abstract(#br)It is well established that homocysteine thiolactone (HTL) is associated with some health disorders, including cardiovascular diseases. HTL is a by-product of sulfur metabolic cycle. So far, its presence has been confirmed in human plasma and urine. It has been also shown that a vast majority of HTL is removed from human body through kidney. Thus, the aim of the current investigations has been the identification, separation and quantification of HTL in urine samples. For the first time a cheap, reliable and robust GC–MS method was developed for the determination of HTL in human urine in the form of its volatile isobutyl chloroformate derivative. Separation of the analyte and internal standard (homoserine lactone (HSL)) was achieved in 15 min followed by mass spectrometry...
全文获取路径: Elsevier  (合作)
影响因子:2.487 (2012)

  • urine 尿
  • determination 测定
  • extraction 提取
  • chloroformate 氯甲酸盐
  • liquid 液体
  • human 人的
  • homocysteine 同型胱氨酸
  • technique 技术
  • Application 应用