AMP-activated protein kinase connects cellular energy metabolism to K ATP channel function
作者: Hidetada YoshidaLi BaoEirini KefaloyianniEylem TaskinUzoma OkorieMiyoun HongPiyali Dhar-ChowdhuryMichiyo KanekoWilliam A. Coetzee
作者单位: 1Pediatric Cardiology, NYU School of Medicine, New York, USA
2Pharmacology, NYU School of Medicine, New York, USA
3Physiology and Neuroscience, NYU School of Medicine, New York, USA
刊名: Journal of Molecular and Cellular Cardiology, 2012, Vol.52 (2), pp.410-418
来源数据库: Elsevier Journal
DOI: 10.1016/j.yjmcc.2011.08.013
关键词: ATP-sensitive K + channelsAMP-activated protein kinasePotassium channelsEnergy metabolismLocal signaling
原始语种摘要: Abstract(#br)AMPK is an important sensor of cellular energy levels. The aim of these studies was to investigate whether cardiac K ATP channels, which couple cellular energy metabolism to membrane excitability, are regulated by AMPK activity. We investigated effects of AMPK on rat ventricular K ATP channels using electrophysiological and biochemical approaches. Whole-cell K ATP channel current was activated by metabolic inhibition; this occurred more rapidly in the presence of AICAR (an AMPK activator). AICAR had no effects on K ATP channel activity recorded in the inside-out patch clamp configuration, but ZMP (the intracellular intermediate of AICAR) strongly activated K ATP channels. An AMPK-mediated effect is demonstrated by the finding that ZMP had no effect on K ATP channels in the...
全文获取路径: Elsevier  (合作)
影响因子:5.148 (2012)