Dose-response effects of gramicidin-D, EDTA, and nonoxynol-9 on sperm motion parameters and acrosome status
作者: Grace M. Centola
作者单位: 1Andrology Laboratory, Division of Reproductive Endocrinology-Infertility, Department of Obstetrics and Gynecology, University of Rochester Medical Center, Rochester, New York, USA
刊名: Contraception, 1998, Vol.58 (1), pp.35-38
来源数据库: Elsevier Journal
DOI: 10.1016/S0010-7824(98)00057-2
关键词: spermgramicidin-Dnonoynol-9sperm acrosome
原始语种摘要: Abstract(#br)Previous reports showed that gramicidin-D (G-D), a polypeptide with antiviral and antimicrobial properties, nonoxynol-9 (N9), a common spermicidal detergent, and EDTA, a Ca-Mg chelating agent, inhibited sperm motility and cervical mucus penetration. The purpose of this study was to determine the dose-response effects of G-D, N9, EDTA and G-D + EDTA on sperm motion parameters and acrosome status. Semen specimens from known fertile donors were subjected to computer-assisted semen analysis of motility, path velocity, progressive velocity, and hyperactivation prior to and after incubation with varying concentrations of gramicidin-D, EDTA and nonoxynol-9. Each specimen was also prepared for acrosome status using rhodamine isothiocyanate conjugated pisum sativum agglutinin...
全文获取路径: Elsevier  (合作)
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影响因子:3.09 (2012)

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