Formulation of the adenylate cyclase toxin of Bordetella pertussis as protein-coated microcrystals
作者: Abdolmajid KhosravaniMarie-Claire ParkerRoger PartonJohn Coote
作者单位: 1Division of Infection and Immunity, Institute of Biomedical and Life Sciences, Glasgow Biomedical Research Centre, University of Glasgow, 120 University Place, Glasgow G12 8TA, UK
2Xstalbio Limited, CIDS, Thomson Building, University Avenue, Glasgow G12 8QQ, UK
刊名: Vaccine, 2007, Vol.25 (22), pp.4361-4367
来源数据库: Elsevier Journal
DOI: 10.1016/j.vaccine.2007.03.035
关键词: Adenylate cyclase toxinVaccine formulationProtein-coated microcrystals
原始语种摘要: Abstract(#br)Adenylate cyclase toxin (CyaA) is an important virulence factor of Bordetella pertussis , the causative agent of whooping cough, and, in its detoxified form, a potential component of acellular pertussis vaccines. This study reports the application of a novel technology, formulation of CyaA as protein-coated microcrystals (PCMC), to improve the performance of CyaA as a vaccine component. CyaA is normally stored in a high urea concentration to prevent aggregation and to maintain stability of the protein. The aim of the work was to stabilise CyaA on a crystalline support to create a dry powder that could be reconstituted in aqueous buffer, free of urea. CyaA, formulated as PCMC with microcrystals of dl -valine, retained full adenylate cyclase (AC) and cell invasive (cytotoxic)...
全文获取路径: Elsevier  (合作)
影响因子:3.492 (2012)