Vaccination of cattle with TickGARD induces cross-reactive antibodies binding to conserved linear peptides of Bm86 homologues in Boophilus decoloratus
作者: David OdongoLucy KamauRobert SkiltonStephen MwauraCordula NitschAnthony MusokeEvans TarachaClaudia DaubenbergerRichard Bishop
作者单位: 1International Livestock Research Institute, PO Box 30709, Nairobi 00100, Kenya
2Department of Medical Parasitolgy and Infection Biology, Molecular Immunology Unit, Swiss Tropical Institute, Socinstrasse 57, PO Box CH-4002, Basel, Switzerland
3Department of Zoology, Kenyatta University, PO Box 43844, Nairobi, Kenya
4Department of Neuroanatomy, Institute of Anatomy, University of Basel, Pestalozzistrasse 20, CH-4056 Basel, Switzerland
刊名: Vaccine, 2006, Vol.25 (7), pp.1287-1296
来源数据库: Elsevier Journal
DOI: 10.1016/j.vaccine.2006.09.085
关键词: TickGARD vaccineEpitope mappingBoophilus ticksSynthetic peptides
原始语种摘要: Abstract(#br)Vaccines based on recombinant Bm86 gut antigen from Boophilus microplus are a useful component of integrated control strategies against B. microplus infestations of cattle. The capacity of such vaccines to control heterologous infestations by two African tick species was investigated. The mean weight of engorged female ticks and mean egg mass per tick were significantly reduced in B. decoloratus infestations, but there was no effect of the vaccine against adult Rhipicephalus appendiculatus . We cloned, sequenced and expressed two Bm86 homologues (Bd86) from B. decoloratus . Amino acid sequence identity between Bd86 homologues (Bd86-1 and Bd86-2) and Bm86 was 86% and 85%, respectively, compared to 93% identity between the variants. Native Bd86 protein in B. decoloratus tick...
全文获取路径: Elsevier  (合作)
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影响因子:3.492 (2012)

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