Osteogenic differentiation of mesenchymal stem cells using PAMAM dendrimers as gene delivery vectors
作者: José Luís SantosElena OramasAna Paula PêgoPedro Lopes GranjaHelena Tomás
作者单位: 1Centro de Química da Madeira, Departamento de Química, Universidade da Madeira, Campus Universitário da Penteada, 9000-390 Funchal, Portugal
2INEB — Instituto de Engenharia Biomédica, Divisão de Biomateriais, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal
3Universidade do Porto, Faculdade de Engenharia, Dep. Eng. Metalúrgica e Materiais, Rua Dr. Roberto Frias s/n, 4200-465 Porto, Portugal
刊名: Journal of Controlled Release, 2008, Vol.134 (2), pp.141-148
来源数据库: Elsevier Journal
DOI: 10.1016/j.jconrel.2008.11.007
关键词: PAMAM dendrimersBone regenerationNonviral gene deliveryMesenchymal stem cellsBone morphogenetic protein
原始语种摘要: Abstract(#br)This paper reports the use of different generations of polyamidoamine (PAMAM) dendrimers for the in vitro transfection of mesenchymal stem cells (MSCs). A systematic study was carried out on the transfection efficiency achieved by the PAMAM dendrimers using a β-galactosidase reporter gene system. Transfection results were shown to be dependent upon the generation of dendrimers, the amine to phosphate group ratio and the cell passage number. In all cases, the transfection efficiency was very low. Nevertheless, it was hypothesized that a low transfection level could be sufficient to promote the in vitro differentiation of MSCs towards the osteoblastic lineage. To address this possibility, dendrimers carrying the human bone morphogenetic protein-2 (hBMP-2) gene-containing...
全文获取路径: Elsevier  (合作)
影响因子:7.633 (2012)