Major metalloprotease gene of Serratia marcescens is conserved and provides a molecular typing method to differentiate clinical isolates
作者: Padman Jayaratne
作者单位: 1Department of Laboratory Medicine, Microbiology Section, Hamilton Civic Hospitals, Hamilton General Division, Hamilton, Ontario L8L 2X2, Canada
刊名: Journal of Microbiological Methods, 1996, Vol.26 (3), pp.261-269
来源数据库: Elsevier Journal
DOI: 10.1016/0167-7012(96)00922-0
关键词: Metalloprotease geneMolecular subtypingSerratia marcescens
原始语种摘要: Abstract(#br)The occurrence and heterogeneity of the 51-kDa major metalloprotease gene from Serratia marcescens which is thought to be involved in pathogenesis was examined using polymerase chain reaction (PCR) and restriction endonuclease analysis (REA). Ten clinical isolates of S. marcescens associated with a previously characterized outbreak and 80 epidemiologically unrelated isolates were investigated. In all isolates, a 1.5-kbp DNA fragment was amplified from the metalloprotease gene. REA of the amplified DNA showed 3 restriction fragment length polymorphism (RFLP) patterns using Hin cII and 5 patterns using Rsa I. Molecular subtyping with the protease gene was performed on 20 epidemiologically unrelated isolates and 10 outbreak-associated isolates. Size-separated genomic DNA...
全文获取路径: Elsevier  (合作)
影响因子:2.161 (2012)