Isolation of single immunohistochemically identified whole neuronal cell bodies from post-mortem human brain for simultaneous analysis of multiple gene expression
作者: Janet E CheethamPaul D ColemanNienwen Chow
作者单位: 1Department of Neurobiology and Anatomy, University of Rochester School of Medicine and Dentistry, Box 603, 601 Elmwood Ave, Rochester, NY 14623, USA
刊名: Journal of Neuroscience Methods, 1997, Vol.77 (1), pp.43-48
来源数据库: Elsevier Journal
DOI: 10.1016/S0165-0270(97)00109-X
关键词: Alzheimer's diseaseHippocampusNeuronImmunohistochemistryAntisense RNADot blot hybridization
原始语种摘要: Abstract(#br)In Alzheimer's disease (AD), one cell in the brain may clearly be affected, while an adjacent cell appears healthy or unaffected. Previous technology has allowed us to examine one message at a time, at the level of a single cell (in situ hybridization, ISH), or multiple messages in a heterogeneous population of cells (Northern analysis). We have developed a methodology to build up a profile of multiple mRNA expression in single, whole, post-mortem cells that have been immunohistochemically (IHC) characterized. Fresh post-mortem tissue is spread into a layer one cell thick and fixed. Neurons are identified using an antibody to neurofilament and isolated using a micropipette. The mRNA is reverse transcribed and PCR carried out to confirm that material is present. A...
全文获取路径: Elsevier  (合作)
影响因子:2.114 (2012)