Target cell specificity of the Pasteurella haemolytica leukotoxin is unaffected by the nature of the fatty-acyl group used to activate the toxin in vitro
作者: Kalantar HormoziRoger PartonJohn Coote
作者单位: 1Division of Infection and Immunity, Institute of Biomedical and Life Sciences, Glasgow University, Joseph Black Building, Glasgow G12 8QQ, UK
刊名: FEMS Microbiology Letters, 1998, Vol.169 (1), pp.139-145
来源数据库: Elsevier Journal
DOI: 10.1016/S0378-1097(98)00476-5
关键词: Toxin acylationLeukotoxin activationPasteurella haemolytica
原始语种摘要: Abstract(#br)The leukotoxin (LktA) of Pasteurella haemolytica is active only against cells of ruminant origin. It is synthesised as an inactive protoxin encoded by the lktA gene and post-translationally modified to the active toxin by the product of the lktC gene. The LktA and LktC proteins were expressed separately in Escherichia coli and partially purified. Active LktA was produced in vitro in the presence of LktC and acyl-acyl carrier protein (ACP) charged separately in vitro with a fatty-acyl group. The toxic activity and target cell specificity of LktA and adenylate cyclase toxin (CyaA), a toxin active against a wide variety of mammalian cells, were investigated after activation with ACP charged with different fatty acids. Palmitoyl-ACP produced the most active toxin in both cases...
全文获取路径: Elsevier  (合作)
影响因子:2.049 (2012)