Derivation of goat embryonic stem cell-like cell lines from in vitro produced parthenogenetic blastocysts
作者: Arun Kumar DeShweta GargDinesh Kumar SinghalHrudananda MalikAyan MukherjeeManoj Kumar JenaSudarshan KumarJai Kumar KaushikAshok Kumar MohantyBikash Chandra DasSadhan BagSubrata Kumar BhanjaDhruba Malakar
作者单位: 1Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana, India
2Veterinary Physiology and Climatology, Indian Veterinary Research Institute, Izatnagar, Bareilly, India
3Central Avian Research Institute, Izatnagar, Bareilly, India
刊名: Small Ruminant Research, 2013, Vol.113 (1), pp.145-153
来源数据库: Elsevier Journal
DOI: 10.1016/j.smallrumres.2013.01.018
关键词: ParthenogenesisEmbryonic stem cell-like cellsBlastocystsGoat
原始语种摘要: Abstract(#br)Parthenogenesis is the biological phenomenon by which embryonic development is initiated without male contribution. Parthenogenesis is a very useful method of derivation of embryonic stem cells (ESCs), which may be an important source of histocompatible cells and tissues for cell therapy. The aim of the present study was to derive and characterize goat embryonic stem cell-like cells from in vitro developed blastocysts following parthenogenetic activation of goat oocytes. Two parthenogenetic embryonic stem cell-like cell (gPESC) lines were established. The gPES cell-like cell colonies showed typical ESC morphology and expressed ESC specific markers such as alkaline phosphatase, Oct-4, Sox-2, Nanog, TRA-1-60, SSEA-4 and TRA-1-81. Genetic stability of the cell lines was proven...
全文获取路径: Elsevier  (合作)
影响因子:1.124 (2012)