Heme degradation upon production of endogenous hydrogen peroxide via interaction of hemoglobin with sodium dodecyl sulfate
作者: N. SalehiA.A. Moosavi-MovahediL. FotouhiS. YousefinejadM. ShourianR. HosseinzadehN. SheibaniM. Habibi-Rezaei
作者单位: 1Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran
2Center of Excellence in Biothermodynamics, University of Tehran, Tehran, Iran
3Departments of Ophthalmology and Visual Sciences and Pharmacology, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA
4Schools of Biology, University of Tehran, Tehran, Iran
刊名: Journal of Photochemistry & Photobiology, B: Biology, 2014, Vol.133 , pp.11-17
来源数据库: Elsevier Journal
DOI: 10.1016/j.jphotobiol.2014.02.014
关键词: HemoglobinSodium dodecyl sulfateHeme degradationEndogenous hydrogen peroxideChemiluminescenceMultivariate curve resolution
英文摘要: Abstract(#br)In this study the hemoglobin heme degradation upon interaction with sodium dodecyl sulfate (SDS) was investigated using UV–vis and fluorescence spectroscopy, multivariate curve resolution analysis, and chemiluminescence method. Our results showed that heme degradation occurred during interaction of hemoglobin with SDS producing three fluorescent components. We showed that the hydrogen peroxide, produced during this interaction, caused heme degradation. In addition, the endogenous hydrogen peroxide was more effective in hemoglobin heme degradation compared to exogenously added hydrogen peroxide. The endogenous form of hydrogen peroxide altered oxyHb to aquamethemoglobin and hemichrome at low concentration. In contrast, the exogenous hydrogen peroxide lacked this ability under...
全文获取路径: Elsevier  (合作)