Phaseolin: A 47.5<ce:hsp sp="0.25"/>kDa protein of red kidney bean ( Phaseolus vulgaris L.) plays a pivotal role in hypersensitivity induction
作者: Sandeep KumarAlok Kumar VermaAkanksha SharmaRuchi RoyDinesh KumarGiridhar BHAnurag TripathiBhushan P. ChaudhariMukul DasS.K. JainPremendra D. Dwivedi
作者单位: 1Food, Drug and Chemical Toxicology Group, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), M.G. Marg, Post Box No. 80, Lucknow 226001, India
2Department of Biotechnology, Faculty of Science, Jamia Hamdard, Hamdard Nagar, New Delhi 110062, India
3Department of TB, Chest & Respiratory Diseases, K S Hegde Medical Academy, Mangalore, India
4Central Pathology Laboratory, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), Gehru Campus, Lucknow, India
刊名: International Immunopharmacology, 2014, Vol.19 (1), pp.178-190
来源数据库: Elsevier Journal
DOI: 10.1016/j.intimp.2014.01.014
关键词: AllergensFood allergyLegumesPeptide mass fingerprintingTranscription factors
原始语种摘要: Abstract(#br)Red kidney bean ( Phaseolus vulgaris L.), a protein rich legume, is consumed globally due to its delicacy. This study was aimed to purify, characterize and assess allergenicity of one of its clinically relevant allergens, later identified as phaseolin. This study was carried out using clinical, in vivo and ex vivo approaches. Phaseolin, an abundant protein of red kidney bean, was purified by column chromatography and reverse-phase-HPLC techniques and characterized by peptide mass fingerprinting. The IgE immunoblotting using red kidney bean allergic patients sera showed phaseolin as a major IgE binding protein of red kidney bean. Phaseolin treated mice demonstrated enhanced levels of specific IgE and IgG1, mouse mast cell protease-1, mRNA expressions of IL-4 , IL-5 , IL-13 and...
全文获取路径: Elsevier  (合作)
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影响因子:2.417 (2012)

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