A fluorescence-based assay for the measurement of S -adenosylhomocysteine hydrolase activity in biological samples
作者: Roman HudecKozo HamadaKatsuhiko Mikoshiba
作者单位: 1Laboratory for Developmental Neurobiology, Brain Science Institute, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan
2Calcium Oscillation Project, ICORP-SORST, Japan Science and Technology Agency, Kawaguchi, Saitama 332-0012, Japan
刊名: Analytical Biochemistry, 2013, Vol.433 (2), pp.95-101
来源数据库: Elsevier Journal
DOI: 10.1016/j.ab.2012.10.014
关键词: S -Adenosylhomocysteine hydrolaseFluorescence-based enzyme-coupled assayBiological samples
英文摘要: Abstract(#br)The methylation of DNA, RNA, and proteins plays crucial roles in numerous biological processes, including epigenetic control, virus replication, and cell differentiation. In mammals, the rate-limiting step of the S -adenosylmethionine-dependent methylation process is exclusively controlled by S -adenosylhomocysteine (S-AdoHcy) hydrolase (SAHH). SAHH hydrolyzes S-AdoHcy to adenosine and homocysteine (Hcy) and is therefore a potential therapeutic target for various diseases, including cancer, malaria, and viral diseases. However, a simple and highly sensitive assay for the evaluation of SAHH activity, particularly for drug discovery, had not yet been developed. Here we present the development of a fluorescence-based assay for the measurement of SAHH activity in biological...
全文获取路径: Elsevier  (合作)
影响因子:2.582 (2012)

  • hydrolase 水解酶
  • assay 试金
  • Fluorescence 荧光
  • based 基于
  • coupled 耦合
  • measurement 测量
  • fluorescence 荧光
  • biological 生物学的
  • enzyme 
  • activity 活度