Developments on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for identifying dissolved and particulate proteins in seawater after two-dimensional sodium dodecyl sulfate–polyacrylamide gel electrophoresis
作者: Natalia García-OteroJana Alonso-LorenzoMaría del Carmen Barciela-AlonsoPilar Bermejo-BarreraAntonio Moreda-Piñeiro
作者单位: 1Department of Analytical Chemistry, Nutrition and Bromatology, Faculty of Chemistry, University of Santiago de Compostela, Avenida das Ciencias, s/n, 15782 Santiago de Compostela, Spain
2Proteomics Facility, Instituto de Investigación Sanitaria de Santiago de Compostela (IDIS), Travesía da Choupana, s/n, 15706 Santiago de Compostela, Spain
刊名: Microchemical Journal, 2015, Vol.122 , pp.50-56
来源数据库: Elsevier Journal
DOI: 10.1016/j.microc.2015.04.011
关键词: Dissolved proteinsPlankton proteinsSeawaterTwo-dimensional sodium dodecyl sulfate–polyacrylamide gel electrophoresisMatrix-assisted laser desorption/ionization time-of-flight mass spectrometry
原始语种摘要: Abstract(#br)Two-dimensional sodium dodecyl sulfate–polyacrylamide gel electrophoresis (2D-SDS–PAGE) was applied to separate protein in dissolved organic matter (DOM) and in particulate organic matter (POM) from seawater. Dissolved proteins were concentrated and purified using tangential flow ultrafiltration (UF) and centrifugal ultrafiltration. Twelve proteins (Sypro Ruby stained 2D-gels) exhibited isoelectric points (pIs) ranging from 3.5 to 6.0, and molecular weights (MWs) within the 10–50kDa range.(#br)Marine plankton were concentrated by centrifugation, and proteins were isolated by grounding the biomass under liquid nitrogen before several washing stages [10% trichloroacetic acid (TCA) in acetone followed by 0.1M ammonium acetate in 80/20...
全文获取路径: Elsevier  (合作)
影响因子:2.879 (2012)