Milk Fat Globule-EGF Factor 8, Secreted by Mesenchymal Stem Cells, Protects Against Liver Fibrosis in Mice
作者: Su Yeon AnYu Jin JangHee-Joung LimJiyou HanJaehun LeeGyunggyu LeeJi Young ParkSeo-Young ParkJi Hyang KimByung-Rok DoChugseong HanHee-Kyung ParkOk-Hee KimMyeong Jun SongSay-June KimJong-Hoon Kim
作者单位: 1Laboratory of Stem Cells and Tissue Regeneration, Department of Biotechnology, College of Life Sciences & Biotechnology, Korea University, Seoul, Republic of Korea
2Biotechnology Research Institute, HurimBioCell Inc., Seoul, Republic of Korea
3Department of Oral Medicine & Oral Diagnosis, Seoul National University Dental Hospital, School of Dentistry & Dental Research Institute, Seoul National University, Seoul, Republic of Korea
4Nexel, CO., Ltd, Seoul, Republic of Korea
5Department of Surgery, Daejeon St. Mary’s Hospital, College of Medicine, the Catholic University of Korea, Daejeon, Republic of Korea
6Division of Hepatology, Department of Internal Medicine, Daejeon St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Daejeon, Republic of Korea
刊名: Gastroenterology, 2016
来源数据库: Elsevier Journal
DOI: 10.1053/j.gastro.2016.12.003
关键词: mouse modelsignal transductionMMPdecorin
英文摘要: Abstract(#br)Background & Aims(#br)Mesenchymal stem cells (MSCs) mediate tissue repair and might be used to prevent or reduce liver fibrosis. However, little is known about the anti-fibrotic factors secreted from MSCs or their mechanisms.(#br)Methods(#br)Umbilical cord-derived MSCs (UCMSCs) were differentiated into hepatocyte-like cells (hpUCMSCs), medium was collected, and secretome proteins were identified and quantified using nanochip-liquid chromatography/quadruple time-of-flight mass spectrometry. Liver fibrosis was induced in mice by intraperitoneal injection of thioacetamide or CCl 4 ; some mice were then given injections of secretomes or proteins. Liver tissues were collected and analyzed by histology or PCR array, to analyze changes in gene expression patterns. We analyzed the...
全文获取路径: Elsevier  (合作)
影响因子:12.821 (2012)