Use of MALDI-TOF mass spectrometry after liquid enrichment (BD Bactec™) for rapid diagnosis of bone and joint infections
作者: Elise LallemandCédric ArvieuxGuillaume CoiffierJean-Louis PolardJean-David AlbertPascal GuggenbuhlAnne Jolivet-Gougeon
作者单位: 1EA 1254 Microbiologie/INSERM NUMECAN, Université de Rennes 1, 2, avenue du Pr Léon Bernard, 35043 Rennes, France
2Pole Biologie, Rennes University Hospital, 35043 Rennes, France
3Service des Maladies Infectieuses, Rennes University Hospital, 2 rue Henri Le Guilloux, 35043 Rennes, France
4Centre de Référence en Infections Ostéo-Articulaires du Grand Ouest (CRIOGO), France
5Service de Rhumatologie, Hôpital Sud, CHU, F-35000 Rennes, France
6INSERM UMR U991/NUMECAN, F-35000 Rennes, France
7Service de Chirurgie orthopédique, Rennes University Hospital, 2 rue Henri Le Guilloux, 35043 Rennes, France
8Université de Rennes 1, F-35000 Rennes, France
刊名: Research in Microbiology, 2016
来源数据库: Elsevier Journal
DOI: 10.1016/j.resmic.2016.09.005
关键词: MALDI-TOF mass spectrometryOsteoarticular infectionSepsityper ® kitTime of detectionBeadmill processingPolymicrobial samples
英文摘要: Abstract(#br)Advantages of MALDI-TOF MS (MS) were evaluated for diagnosis of bone and joint infections after enrichment of synovial fluid (SF) or crushed osteoarticular samples (CSs). MS was performed after enrichment of SF or crushed osteoarticular samples CS (n = 108) in both aerobic and anaerobic vials. Extraction was performed on 113 vials (SF: n = 47; CS: n = 66), using the Sepsityper ® kit prior identification by MS. The performances of MS, score and reproducibility results on bacterial colonies from blood agar and on pellets after enrichment in vials, were compared. MS analysis of the vial resulted in correct identification of bacteria at a species and genus level (80.5% and 92% of cases, respectively). The reproducibility was superior for aerobic Gram-positive bacteria (...
全文获取路径: Elsevier  (合作)
影响因子:2.889 (2012)