Characterizations of protein-ligand reaction kinetics by transistor-microfluidic integrated sensors
作者: Chun-Ho ChouJin-Chun LimYao-Hsuan LaiYung-Tsan ChenYu-Hwa LoJian-Jang Huang
作者单位: 1Graduate Institute of Photonics and Optoelectronics, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 106, Taiwan
2Materials Science and Engineering Program, University of California San Diego, La Jolla, CA, 92093-0418, United States
3Electrical and Computer Engineering Department, University of California San Diego, La Jolla, CA, 92093-0407, United States
4Department of Electrical Engineering, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd., Taipei, 10617, Taiwan
刊名: Analytica Chimica Acta, 2020, Vol.1110 , pp.1-10
来源数据库: Elsevier Journal
DOI: 10.1016/j.aca.2020.03.012
关键词: MicrofluidicIGZO Thin-film transistor biosensorProtein detection
英文摘要: Abstract(#br)Understanding the binding affinities and kinetics of protein-ligand interactions using a label-free method is crucial for identifying therapeutic candidates in clinical diagnostics and drug development. In this work, the IGZO-TFT (thin-film transistor) biosensor integrated with a tailored microfluidic chip was developed to explore binding kinetics of protein-ligand biochemical interactions in the real-time manner. The IGZO-TFT sensor extracts the binding characteristics through sensing biomolecules by their electrical charges. Using lysozyme and tri-N-acetyl-D-glucosamine (NAG 3 ) as an example, we established a procedure to obtain the parameters, such as the dissociation constant, K d , and association rate constant, k a , that are critical to biochemical reactions. The...
全文获取路径: Elsevier  (合作)
影响因子:4.387 (2012)

  • reaction 反酌
  • kinetics 动力学
  • protein 蛋白质
  • transistor 晶体管
  • integrated 综[组,复]合
  • ligand 配合体