Tuning liposome composition to modulate corona formation in human serum and cellular uptake
作者: Keni YangBárbara MesquitaPeter HorvatovichAnna Salvati
作者单位: 1Department of Pharmacokinetics, Toxicology and Targeting, Groningen Research Institute of Pharmacy, University of Groningen, A. Deusinglaan 1, 9713AV Groningen, the Netherlands
2Department of Analytical Biochemistry, Groningen Research Institute of Pharmacy, University of Groningen, A. Deusinglaan 1, 9713AV Groningen, the Netherlands
刊名: Acta Biomaterialia, 2020, Vol.106 , pp.314-327
来源数据库: Elsevier Journal
DOI: 10.1016/j.actbio.2020.02.018
关键词: LiposomesNanoparticle coronaHuman serumCellular uptake
原始语种摘要: Abstract(#br)Nano-sized objects such as liposomes are modified by adsorption of biomolecules in biological fluids. The resulting corona critically changes nanoparticle behavior at cellular level. A better control of corona composition could allow to modulate uptake by cells. Within this context, in this work, liposomes of different charge were prepared by mixing negatively charged and zwitterionic lipids to different ratios. The series obtained was used as a model system with tailored surface properties to modulate corona composition and determine the effects on liposome interactions with cells. Uptake efficiency and uptake kinetics of the different liposomes were determined by flow cytometry and fluorescence imaging. Particular care was taken in optimizing the methods to isolate the...
全文获取路径: Elsevier  (合作)
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来源刊物:
影响因子:5.093 (2012)

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关键词翻译
关键词翻译
  • corona 反应边
  • uptake 上风口
  • kinetics 动力学
  • serum 血清
  • adsorb 吸附
  • biological 生物学的
  • zwitterionic 两性离子的
  • nanoparticle 乳酸
  • formulation 配方
  • human 人的