Early diagnosis of experimental Trichinella spiralis infection by nano-based enzyme-linked immunosorbent assay (nano-based ELISA)
作者: Maha M. Gomaa
作者单位: 1Department of Medical Parasitology, Faculty of Medicine, Alexandria University, Alexandria, Egypt
刊名: Experimental Parasitology, 2020, Vol.212
来源数据库: Elsevier Journal
DOI: 10.1016/j.exppara.2020.107867
关键词: Trichinella spiralisCAg antigenStandard ELISAGold nano-based ELISAExperimental infection
原始语种摘要: Abstract(#br)Trichinellosis is a serious foodborne zoonotic disease. It is an important threat to public health all over the world. Although anti- Trichinella IgG detection is the most widely used method for diagnosis of trichinellosis, but there is an obvious window between clinical symptoms and positive serology. Gold nanoparticles (AuNPs) can be conjugated with antibodies affording them promising applications for bio-chemical detection. Herein, AuNPs-based ELISA was evaluated for the first time in the detection of Trichinella spiralis circulating antigen (CAg) for its potential as a diagnostic tool of experimental infection. Swiss Albino mice were orally inoculated with 100 muscle larvae/mouse. Animals were sacrificed 6, 8, 10, 12, 14, 16, 22 and 28 day-post infection (dpi). Blood...
全文获取路径: Elsevier  (合作)
影响因子:2.154 (2012)

  • immunosorbent 免疫吸收剂
  • spiralis 腋素
  • ELISA 酶联免疫吸收分析
  • diagnosis 特镇述
  • experimental 实验的
  • infection 感染
  • based 基于
  • enzyme 
  • linked 连接的
  • assay 试金