Leucine rich repeats and calponin homology domain containing 1 inhibits NK-92 cell cytotoxicity through attenuating Src signaling
富含亮氨酸重复序列和含1的calponin同源结构域通过减弱Src信号抑制NK-92细胞毒性
作者: Kai DaiZubing ChenSha SheJinzhi ShiJiling ZhuYabing Huang
作者单位: 1Department of Infectious Diseases, Renmin Hospital of Wuhan University, Wuhan, 430060, China
2Department of Hepatobiliary Surgery, Renmin Hospital of Wuhan University, Wuhan, 430060, China
3Department of Pathology, Renmin Hospital of Wuhan University, Wuhan, 430060, China
刊名: Immunobiology, 2020
来源数据库: Elsevier Journal
DOI: 10.1016/j.imbio.2020.151934
关键词: Leucine rich repeats and calponin homology domain containing 1Natural killer cellsCytotoxicityImmunotherapy
原始语种摘要: Abstract(#br)NK-92 cell line has been used as anti-tumor cytotoxic effector cells in immunotherapy. Leucine-rich repeats and calponin homology domain containing 1 (LRCH1) is a novel gene of which the function is unclear. In the present study, we investigated the role of LRCH1 in NK-92 cell cytotoxicity. LRCH1 was ablated in NK-92 cells through CRISP-Cas9-mediated knockout. LRCH1 knockout did not influence the basal behavior of NK-92 cells such as cell survival, expression of natural cytotoxicity receptors, and proliferation. However, upon the contact with tumor cells, LRCH1 knockout promoted NK-92 cell cytotoxicity to tumor cells. Besides, LRCH1 knockout increased the production of cytotoxic mediators such as IFN-γ, TNF-α, IL-2, and granzyme B in NK-92 cells after tumor cell contact....
全文获取路径: Elsevier  (合作)
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影响因子:2.814 (2012)

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关键词翻译
关键词翻译
  • cytotoxicity 细胞毒性
  • knockout 出模器
  • attenuating 衰减的
  • domain 领域
  • homology 同源
  • cells 麻风细胞
  • tumoricidal 破坏瘤的
  • engagement 啮合
  • signaling 发信号
  • killer 载劣扼杀剂