Bacterial membrane permeability of antimicrobial polymethacrylates: Evidence for a complex mechanism from super-resolution fluorescence imaging
抗菌剂聚甲基丙烯酸甲酯的细菌膜通透性:超分辨荧光成像对复杂机制的证据
作者: Thomas D. MichlBen HibbsLauren HydeAlmar PostmaDung Thuy Thi TranAigerim ZhalgasbaikyzyKrasimir VasilevLaurence MeagherHans J. GriesserKatherine E.S. Locock
作者单位: 1School of Engineering, University of South Australia, Mawson Lakes Blvd, Mawson Lakes, SA 5095, Australia
2Materials Characterisation and Fabrication Platform, The University of Melbourne, Parkville, VIC 3010, Australia
3CSIRO Manufacturing, Research Way, Clayton, VIC 3168, Australia
4Department of Materials Science and Engineering, Monash University, Clayton, VIC 3168, Australia
5Future Industries Institute, University of South Australia, Mawson Lakes Blvd, Mawson Lakes, SA 5095, Australia
6Department of Chemical Engineering, The University of Melbourne, Parkville, VIC 3010, Australia
刊名: Acta Biomaterialia, 2020
来源数据库: Elsevier Journal
DOI: 10.1016/j.actbio.2020.03.011
关键词: Antimicrobial polymerRAFTSuper-resolution imagingOMXFluorescent tagRhodamine RAFT agentMechanismDNA binding
原始语种摘要: Abstract(#br)Amphiphilic polymers bearing cationic moieties are an emerging alternative to traditional antibiotics given their broad-spectrum activity and low susceptibility to the development of resistance. To date, however, much remains unclear regarding their mechanism of action. Using functional assays (ATP leakage, cell viability, DNA binding) and super-high resolution structured illumination microscopy (OMX-SR) of fluorescently tagged polymers, we present evidence for a complex mechanism, involving membrane permeation as well as cellular uptake, interaction with intracellular targets and possible complexation with bacterial DNA.(#br)Statement of Significance(#br)This manuscript details the first study to systematically and directly investigate the mechanism of action of...
全文获取路径: Elsevier  (合作)
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影响因子:5.093 (2012)

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关键词翻译
关键词翻译
  • mechanism 机构
  • antimicrobial 杀菌剂
  • imaging 图像形成
  • functional 功能的
  • complex 超群
  • fluorescence 荧光
  • binding 装订
  • membrane 
  • bacterial 细菌的
  • super