Nanoparticle reinforced bacterial outer-membrane vesicles effectively prevent fatal infection of carbapenem-resistant Klebsiella pneumoniae
纳米颗粒增强细菌外膜囊泡有效预防耐碳青霉烯肺炎克雷伯菌致命感染
作者: Guangxi WuHaiying JiXiaoyu GuoYongyong LiTianbin RenHaiqing DongJingxian LiuYiqiong LiuXueyin ShiBin He
作者单位: 1Department of Anesthesiology and SICU, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China
2Institute for Biomedical Engineering & Nano Science, Tongji University School of Medicine, Shanghai, China
3Department of Clinical Laboratory, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China
刊名: Nanomedicine: Nanotechnology, Biology, and Medicine, 2020, Vol.24
来源数据库: Elsevier Journal
DOI: 10.1016/j.nano.2019.102148
关键词: NanovaccineAlbumin nanoparticlesCarbapenem-resistant Klebsiella pneumoniaeOuter membrane vesicles
英文摘要: Abstract(#br)Infection resulting from carbapenem-resistant Klebsiella pneumoniae (CRKP) is an intractable clinical problem. Outer membrane vesicles (OMVs) from CRKP are believed to be potential vaccine candidates. However, their immune response remains elusive due to low structural stability and poor size homogeneity. In this study, hollow OMVs were reinforced internally by size-controlled BSA nanoparticles to obtain uniform and stable vaccines through hydrophobic interaction. The result showed that the BSA-OMV nanoparticles (BN-OMVs) were homogenous with a size around 100 nm and exhibited a core-shell structure. Remarkably, subcutaneous BN-OMVs vaccination mediated significantly higher CRKP specific antibody titers. The survival rate of the mice infected with a lethal dose of CRKP was...
全文获取路径: Elsevier  (合作)
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关键词翻译
关键词翻译
  • resistant 耐久的
  • carbapenem 氨基甲酰
  • effectively 有效地
  • bacterial 细菌的
  • prevent 避免
  • infection 感染
  • membrane 
  • Albumin 清蛋白
  • fatal 致命的
  • reinforced 加筋的