Compact optical detector utilizing light emitting diodes, 50 nL L-shaped silica capillary cell and CCD spectrometer for simultaneous multi-wavelength monitoring of absorbance and fluorescence in microcolumn liquid chromatography
作者: Jozef ŠestákJosef PlanetaVladislav Kahle
作者单位: 1Institute of Analytical Chemistry of the Czech Academy of Sciences, Veveří 97, Brno, Czech Republic
刊名: Analytica Chimica Acta, 2020, Vol.1112 , pp.80-91
来源数据库: Elsevier Journal
DOI: 10.1016/j.aca.2020.03.020
关键词: Simultaneous absorbance-fluorescence detectionMicrocolumn liquid chromatographyMiniaturised detection cellLight emitting diodesCCD spectrometer
原始语种摘要: Abstract(#br)Optical detection setup utilizing light emitting diodes (LEDs), 50 nL L-shaped silica capillary detection cell (L-cell), and low-cost CCD spectrometer is described in this work. Experimental configuration can be equipped with two different LEDs for absorbance measurement and other two LEDs for fluorescence excitation. This setup is capable of simultaneous multi-wavelength monitoring of absorbance and fluorescence when light produced by the individual LEDs and light emitted by the fluorescent analytes is resolved in the spectrum outputted by the CCD spectrometer. Effective optical path of the 0.25 μm I. D. L-cell is 1 mm. Absorbance baseline noise is 1 mAU due to use of low-cost and relatively noisy CCD spectrometer and LED drivers. Nevertheless, the setup can detect adenosine...
全文获取路径: Elsevier  (合作)
影响因子:4.387 (2012)

  • simultaneous 同时的
  • detector 探测器检波器
  • optical 光学的
  • emitting 发出
  • absorbance 吸光度
  • silica 硅土
  • light 
  • chromatography 色层分离法
  • monitoring 监视
  • liquid 液体