Comparison of a glycoprotein E-based ELISA with a varicella-zoster whole-virus ELISA for the quantification of varicella vaccine immune responses in young children
作者: Muriel FeyssaguetValérie BertholdLaurent HelleMichael PoveyStéphanie RavaultStéphane CarrynPaul GillardEmmanuel Di Paolo
作者单位: 1GSK, Rue de l’Institut 89, 1330 Rixensart, Belgium
2GSK, Av. Fleming 20, 1300 Wavre, Belgium
刊名: Vaccine, 2020, Vol.38 (17), pp.3300-3304
来源数据库: Elsevier Journal
DOI: 10.1016/j.vaccine.2020.03.009
关键词: Varicella-zoster virusAntibody assayELISAGlycoprotein EVaricella vaccinationChildren
英文摘要: Abstract(#br)Antibody response against varicella-zoster virus (VZV) is frequently assessed by whole-virus- (anti-VZV) or glycoprotein-based ELISAs. This study compared antibody concentrations measured by an assay quantifying anti-VZV glycoprotein E (anti-gE) and anti-VZV ELISA in 12–23-month-olds, receiving two varicella vaccine doses in a phase III trial (NCT02570126). Samples (pre- and 42 days post-each vaccination) initially tested with anti-VZV ELISA were re-tested with anti-gE ELISA. Of 1138 samples from 397 children, 757 were positive by anti-VZV (antibody concentration ≥25 mIU/mL) and 758 by anti-gE ELISA (≥97 mIU/mL). There were 375 double-negative and only 11 discrepant samples. The overall agreement was 99.03% (95% confidence interval: 98.28–99.52; McNemar p-value = 1). The...
全文获取路径: Elsevier  (合作)
影响因子:3.492 (2012)

  • varicella 水痘
  • zoster 带状疱疹
  • glycoprotein 糖蛋白
  • ELISA 酶联免疫吸收分析
  • immune 免疫的
  • virus 病毒
  • quantification 量化
  • vaccination 注射疫苗
  • whole 全部的
  • vaccine 菌苗