High-sensitive sensing of plant microRNA by integrating click chemistry with an unusual on-bead poly(T)-promoted transcription amplification
作者: Gaoting WangWenjiao FanWei RenXiaoling LiuChenghui Liu
作者单位: 1Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry & Chemical Engineering, Shaanxi Normal University, Xi′an, 710119, Shaanxi Province, PR China
刊名: Analytica Chimica Acta, 2020, Vol.1111 , pp.16-22
来源数据库: Elsevier Journal
DOI: 10.1016/j.aca.2020.03.036
关键词: Plant microRNAClick chemistryTranscription amplificationT7 RNA polymeraseMagnetic beads
原始语种摘要: Abstract(#br)MicroRNAs (miRNAs) act as pivotal regulators in plants. Therefore, sensing strategies with high specificity and high sensitivity are desired for plant miRNA analysis in order to unveil the exact biofunctions of miRNAs. Toward this goal, a fluorescent assay is developed based on a two-step signal amplification strategy. In the first step, target miRNA-templated cycling click nucleic acid ligation is employed for target recognition and amplification, the product of which can bind to magnetic microbeads (MBs) and introduce the T7 promoter sequence to the surface. In the second step, the poly(T) containing transcription template partially hybridizes with the T7 promoter sequence on the ligated strand and then regulates the on-bead transcription in a cycling manner with the...
全文获取路径: Elsevier  (合作)
影响因子:4.387 (2012)

  • unusual 稀有
  • click 掣子
  • transcription 地名转写
  • amplification 放大
  • sensitive 灵敏的
  • integrating 集成
  • chemistry 化学
  • sensing 感觉
  • plant 植物
  • poly