A rapid, simple and highly sensitive UPLC-MS/MS method for quantitation of pimavanserin in plasma and tissues: Application to pharmacokinetics and brain uptake studies in mice
作者: Essam EzzeldinMuzaffar IqbalYousif A. AsiriAzza A. AliToqa El-Nahhas
作者单位: 1Department of Pharmaceutical Chemistry and Drug Bioavailability Laboratory, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia
2National Organization for Drug Control and Research, Cairo, Egypt
3Clinical Pharmacy Department, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia
4Pharmacology and Toxicology Department, Faculty of Pharmacy (Girls) Al-Azhar University, Cairo, Egypt
刊名: Journal of Chromatography B, 2020, Vol.1143
来源数据库: Elsevier Journal
DOI: 10.1016/j.jchromb.2020.122015
关键词: PimavanserinUPLC-MS/MSPharmacokineticsParkinson's diseaseMiceBrain
原始语种摘要: Abstract(#br)Pimavanserin is a new drug approved by the FDA for Parkinson's disease psychosis and other neurological disorders such as Alzheimer's disease. In this study, we developed a UPLC-MS/MS method to quantify pimavanserin disposition in the brain and its pharmacokinetics in mice. Vilazodone was used as the internal standard. Pimavanserin and IS were extracted by liquid-liquid extraction using tert -butyl methyl ether and separated using an Acquity UPLC BEH™ C 18 column. The mobile phase consisted of solvent A (0.1% formic acid in acetonitrile) and B (0.1% formic acid in 20 mM ammonium acetate buffer) (A: B, 70:30 v/v) at a flow rate of 0.25 ml/min. The multiple reaction monitoring transitions were performed at m / z 428.23 → 98.15 for pimavanserin and m / z 441.70 > 155.03 for the...
全文获取路径: Elsevier  (合作)
影响因子:2.487 (2012)

  • plasma 深绿玉髓
  • uptake 上风口
  • quantitation 定量
  • brain 
  • sensitive 灵敏的
  • highly 高度地
  • Application 应用
  • simple 单纯的
  • disease 
  • method 方法