Ubiquitination of DNA Damage-Stalled RNAPII Promotes Transcription-Coupled Repair
DNA损伤-停滞RNAPII泛素化促进转录-偶联修复
作者: Yuka NakazawaYuichiro HaraYasuyoshi OkaOkiru KomineDiana van den HeuvelChaowan GuoYasukazu DaigakuMayu IsonoYuxi HeMayuko ShimadaKana KatoNan JiaSatoru HashimotoYuko KotaniYuka MiyoshiMiyako TanakaAkira SobueNorisato MitsutakeTakayoshi SuganamiAkio MasudaKinji OhnoShinichiro NakadaTomoji MashimoKoji YamanakaMartijn S. LuijsterburgTomoo Ogi
作者单位: 1Department of Genetics, Research Institute of Environmental Medicine (RIeM), Nagoya University, Nagoya, Japan
2Department of Human Genetics and Molecular Biology, Nagoya University Graduate School of Medicine, Nagoya, Japan
3Department of Neuroscience and Pathobiology, Research Institute of Environmental Medicine (RIeM), Nagoya University, Nagoya, Japan
4Department of Neuroscience and Pathobiology, Nagoya University Graduate School of Medicine, Nagoya, Japan
5Department of Human Genetics, Leiden University Medical Center (LUMC), Leiden, the Netherlands
6Frontier Research Institute for Interdisciplinary Sciences, Tohoku University, Sendai, Japan
7Graduate School of Life Sciences, Tohoku University, Sendai, Japan
8Institute of Experimental Animal Sciences, Graduate School of Medicine, Osaka University, Osaka, Japan
9Genome Editing Research and Development (R&D) Center, Graduate School of Medicine, Osaka University, Osaka, Japan
10Department of Molecular Medicine and Metabolism, Research Institute of Environmental Medicine (RIeM), Nagoya University, Nagoya, Japan
11Department of Immunometabolism, Nagoya University Graduate School of Medicine, Nagoya, Japan
12Department of Radiation Medical Sciences, Atomic Bomb Disease Institute, Nagasaki University, Nagasaki, Japan
13Division of Neurogenetics, Center for Neurological Diseases and Cancer, Nagoya University Graduate School of Medicine, Nagoya, Japan
14Department of Bioregulation and Cellular Response, Graduate School of Medicine, Osaka University, Osaka, Japan
15Institute for Advanced Co-Creation Studies, Osaka University, Osaka, Japan
16Division of Animal Genetics, Laboratory Animal Research Center, Institute of Medical Science, The University of Tokyo, Tokyo, Japan
刊名: Cell, 2020, Vol.180 (6), pp.1228-1244.e24
来源数据库: Elsevier Journal
DOI: 10.1016/j.cell.2020.02.010
关键词: RNA polymerase IIRNAPIIUbiquitination of RNAPIITranscription coupled repairTCRNucleotide excision repairNERCockayne syndromeCSUV-sensitive syndromeUVsSChIP-seqUVSSACRL/CSA/CSBTFIIH
原始语种摘要: Summary(#br)Transcription-coupled nucleotide excision repair (TC-NER) is initiated by the stalling of elongating RNA polymerase II (RNAPIIo) at DNA lesions. The ubiquitination of RNAPIIo in response to DNA damage is an evolutionarily conserved event, but its function in mammals is unknown. Here, we identified a single DNA damage-induced ubiquitination site in RNAPII at RPB1-K1268, which regulates transcription recovery and DNA damage resistance. Mechanistically, RPB1-K1268 ubiquitination stimulates the association of the core-TFIIH complex with stalled RNAPIIo through a transfer mechanism that also involves UVSSA-K414 ubiquitination. We developed a strand-specific ChIP-seq method, which revealed RPB1-K1268 ubiquitination is important for repair and the resolution of transcriptional...
全文获取路径: Elsevier  (合作)
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影响因子:31.957 (2012)

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关键词翻译
关键词翻译
  • DNA Deoxyribonucleic Acid
  • nucleotide 核苷酸
  • stalled 失速的
  • protect 庇护
  • together 共同
  • mechanism 机构
  • transcription 地名转写
  • strand 海滨
  • excision 切除
  • coupled 耦合