circINSR Promotes Proliferation and Reduces Apoptosis of Embryonic Myoblasts by Sponging miR-34a
作者: Xuemei ShenXiaoyan ZhangWenxiu RuYongzhen HuangXianyong LanChuzhao LeiHong Chen
作者单位: 1Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, Shaanxi, China
刊名: Molecular Therapy - Nucleic Acids, 2020, Vol.19 , pp.986-999
来源数据库: Elsevier Journal
DOI: 10.1016/j.omtn.2019.12.032
关键词: Muscle developmentBovineCircular RNAsProliferationMiR-34a
原始语种摘要: As a diverse and abundant class of endogenous RNAs, circular RNAs (circRNAs) participate in processes including cell proliferation and apoptosis. Nevertheless, few researchers have investigated the function of circRNAs in bovine muscle development. Based on existing sequencing data, we identified circINSR. The localization of circINSR in bovine myoblasts was investigated by fluorescence in situ hybridization. Molecular and biochemical assays were used to confirm the role of circINSR in myoblast proliferation and the cell cycle. Mitochondrial membrane potential and annexin V-PE/7-AAD staining assays were performed to assess cell apoptosis. Additionally, interactions between circINSR, miR-34a, and target mRNAs were examined using bioinformatics, a luciferase assay, and RNA...
全文获取路径: Elsevier  (合作)