Efficient keratinase expression via promoter engineering strategies for degradation of feather wastes
通过启动子工程策略高效表达角蛋白酶降解羽毛废弃物
作者: Jin-Song GongJin-Peng YeLi-Yan TaoChang SuJiufu QinYan-Yan ZhangHeng LiHui LiZheng-Hong XuJin-Song Shi
作者单位: 1Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Pharmaceutical Sciences, Jiangnan University, Wuxi 214122, PR China
2National Engineering Laboratory for Cereal Fermentation Technology, School of Biotechnology, Jiangnan University, Wuxi 214122, PR China
3Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark
4Jiangsu Provincial Research Center for Bioactive Product Processing Technology, Jiangnan University, Wuxi 214122, PR China
刊名: Enzyme and Microbial Technology, 2020, Vol.137
来源数据库: Elsevier Journal
DOI: 10.1016/j.enzmictec.2020.109550
关键词: KeratinaseExpressionPromoter screeningFermentationBiodegradation
原始语种摘要: Abstract(#br)Keratinases are promising alternatives over ordinary proteases in several industrial applications due to their unique properties compared with their counterparts in the protease categories. However, their large-scale industrial application is limited by the low expression and poor fermentation efficiency of keratinase. Here, we demonstrate that the expression level of keratinase can be improved by constructing a more efficient enzyme expression system hereby enables the highest production titer as regarding recombinant keratinase production to date. Specially, ten promoters were evaluated and the aprE promoter exhibits a significant promotion of keratinase (kerBv) titer from 165 U/mL to 2605 U/mL in Bacillus subtilis . The batch fermentation mode resulted in a maximum...
全文获取路径: Elsevier  (合作)
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影响因子:2.592 (2012)

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关键词翻译
关键词翻译
  • 一批 滴定率
  • keratinase 角朊酶
  • expression 表示
  • feather 羽饰斑
  • degradation 减嚣夷酌
  • promoter 催化剂
  • fermenter 发酵槽
  • titer 滴定率
  • batch 滴定率
  • alternatives 代替案
  • fermentation 发酵