Progesterone's role in deep infiltrating endometriosis: Progesterone receptor and estrogen metabolism enzymes expression and physiological changes in primary endometrial stromal cell culture
孕酮在深层浸润性子宫内膜异位症中的作用:原代子宫内膜基质细胞培养中孕酮受体和雌激素代谢酶的表达及生理变化
作者: Gil KamergorodskyAdriana L. InvittiPaulo D'AmoraRafael M. ParreiraAlexander KopelmanTatiana C.S. BonettiManoel J.B.C. GirãoEduardo Schor
作者单位: 1Pelvic Pain and Endometriosis Unit - Gynecology Department - Escola Paulista de Medicina da Universidade Federal de São Paulo (EPM-UNIFESP) , São Paulo, Brazil
2Cellular and Molecular Gynecology Laboratory - Gynecology Department - Escola Paulista de Medicina da Universidade Federal de São Paulo (EPM-UNIFESP) , São Paulo, Brazil
3General Gynecology Discipline - Gynecology Department, Escola Paulista de Medicina da Universidade Federal de São Paulo (EPM-UNIFESP) , São Paulo, Brazil
刊名: Molecular and Cellular Endocrinology, 2020, Vol.505
来源数据库: Elsevier Journal
DOI: 10.1016/j.mce.2020.110743
关键词: Progesterone receptorsMicrophysiometryGene expressionDeep infiltrating endometriosis
英文摘要: Abstract(#br)To study progesterone signaling activation, we measured changes in extracellular pH as a reflection of Na+/H+ exchange (NHE) using a cytosensor microphysiometer and assessed progesterone receptor (PR) and estrogen metabolism enzymes mRNA expression in cultured endometrial cells from women with deep infiltrating endometriosis and healthy controls using real-time quantitative PCR. This study was conducted at a University hospital and included patients with and without deep infiltrating endometriosis (DIE). Primary endometrial stromal cells (ECs) from women with DIE and controls were treated with 17β-estradiol and progesterone prior to microphysiometer measurements and qPCR evaluations. Decreased progesterone responsiveness and decreased total nuclear PR and HSD17B1 mRNA...
全文获取路径: Elsevier  (合作)
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影响因子:4.039 (2012)

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关键词翻译
关键词翻译
  • endometriosis 子宫内膜异位
  • infiltrating 浸润的
  • endometrial 子宫内膜的
  • estrogen 雌激素
  • stromal 基质的
  • receptor 接受体
  • enzymes 酶类
  • metabolism 代谢
  • expression 表示
  • physiological 生理的