Development of HPLC method for estimation of glyoxylic acid after pre-column fluorescence derivatization approach based on thiazine derivative formation: A new application in healthy and cardiovascular patients’ sera
作者: Marwa F.B. AliNaoya KishikawaNaotaka Kuroda
作者单位: 1Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Assiut University, 71526 Assiut, Egypt
2Course of Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan
刊名: Journal of Chromatography B, 2020, Vol.1143
来源数据库: Elsevier Journal
DOI: 10.1016/j.jchromb.2020.122054
关键词: Glyoxylic acid2-AminobenzenthiolHigh Performance Liquid Chromatography (HPLC)Pre-column fluorescence derivatizationSerum analysis
原始语种摘要: Abstract(#br)Glyoxylic acid (GA) is the intermediate metabolite in various mammalian metabolic pathways. GA showed high reactivity towards formation of advanced glycation end-products (AGEs); the main cause of pathogenesis and complications of many diseases. The presented study aimed to detect GA in healthy and cardiovascular patients’ (CV) sera; however analysis of GA in biological fluid is a challenge and requires chemical derivatization. Hence, a new, highly sensitive, time saving and reproducible pre-column fluorescence derivatization procedure coupled with high performance liquid chromatography (HPLC) method was developed. The derivatization method was based on reaction of 2-aminobenzenthiol (2-ABT), a fluorogenic reagent, with GA in acidic medium to form highly fluorescent thiazine...
全文获取路径: Elsevier  (合作)
影响因子:2.487 (2012)

  • 在之后 健康的
  • derivatization 衍生作用
  • sera 血清
  • fluorescence 荧光
  • derivative 衍生物
  • estimation 估计
  • column 
  • thiazine 噻嗪
  • based 基于
  • healthy 健康的
  • after 健康的