Cyclodextrin and its derivatives enhance protein crystallization by grafted on crystallization plates
作者: Qian-Jin WangGang ZhaoChen-Yan Zhang
作者单位: 1School of Chemical Engineering, Shaanxi Energy Institute, Xianyang 712000, Shaanxi, PR China
2Breast Surgery, The First Hospital of Jilin University, Changchun 130021, Jilin, PR China
3Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi’an 710072, Shaanxi, PR China
刊名: Journal of Crystal Growth, 2020, Vol.536
来源数据库: Elsevier Journal
DOI: 10.1016/j.jcrysgro.2020.125591
关键词: A1. Protein crystallizationB1. CyclodextrinA1. Nucleation
英文摘要: Abstract(#br)Protein drugs are attracting increasing attention. Eight of best-selling drugs in worldwide are proteins. Crystallization is an important protein purification method; however, it remains a bottleneck step. Heterogeneous nucleate is an effective method of enhancing protein crystallization. Cyclodextrin has been widely used in pharmaceutics, and our previous study showed that it is an effective heterogeneous nucleate for enhancing protein crystallization, and it may be widely used in purification of protein drugs. However, cyclodextrin must be added into the crystallization plate wells by hand, which is labor intensive and is not beneficial to automated crystallization screening. We grafted β-cyclodextrin (β-CD) and its derivatives (i.e., p-toluenesulfonyl-β-cyclodextrin...
全文获取路径: Elsevier  (合作)
影响因子:1.552 (2012)

  • protein 蛋白质
  • enhance 增强
  • derivatives 派生物