Biopartitioning micellar chromatography under different conditions: Insight into the retention mechanism and the potential to model biological processes
作者: Fotios TsopelasPanagiotis DaniasAthina PappaAnna Tsantili-Kakoulidou
作者单位: 1Laboratory of Inorganic and Analytical Chemistry, School of Chemical Engineering, National Technical University of Athens, Iroon Polytechniou 9, 157 80 Athens, Greece
2Department of Pharmaceutical Chemistry, School of Pharmacy, National and Kapodistrian University of Athens, Panepistimiopolis, Zografou, 157 71 Athens, Greece
刊名: Journal of Chromatography A, 2020, Vol.1621
来源数据库: Elsevier Journal
DOI: 10.1016/j.chroma.2020.461027
关键词: Biopartitioning micellar chromatographyCell permeabilityHuman oral absorption (%HOA)Plasma protein binding (%PPB)LipophilicityOctanol- water partitioning
原始语种摘要: Abstract(#br)In the present study, 88 structurally- diverse drugs were investigated by biopartitioning micellar chromatography (BMC) using Brij-35 as surfactant under different chromatographic conditions. It was found that temperature and presence of NaCl have only a minor effect in BMC retention. Correlation of BMC retention factors with octanol-water partitioning required the inclusion of fractions of ionized species as additional parameters, showing that there is a weaker effect of ionization in BMC environment. Compared to Immobilized Artificial Membrane (IAM) Chromatography, BMC retention factors cover a relatively narrow span, two-fold smaller than retention factors on IAM stationary phases as a result of the presence of micelles facilitating elution of lipophilic compounds and the...
全文获取路径: Elsevier  (合作)
影响因子:4.612 (2012)

  • retention 保留
  • chromatography 色层分离法
  • partitioning 分块
  • water 
  • octanol 辛醇
  • stationary 固定的
  • micellar 胶束的
  • conditions 条件式
  • structurally 在结构上
  • elution 洗提