miR-25 Promotes Cardiomyocyte Proliferation by Targeting FBXW7
mi R-25通过靶向FBXW7促进心肌细胞增殖
作者: Bei WangMengting XuMiaomiao LiFujian WuShijun HuXiangbo ChenLiqun ZhaoZheyong HuangFeng LanDong LiuYongming Wang
作者单位: 1State Key Laboratory of Genetic Engineering, School of Life Sciences, Zhongshan Hospital, Fudan University, Shanghai 200438, China
2School of Life Sciences, Co-innovation Center of Neuroregeneration, Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong 226001, China
3Beijing Anzhen Hospital, Beijing Institute of Heart Lung and Blood Vessel Disease, Capital Medical University, Beijing 100029, China
4Institute for Cardiovascular Science & Department of Cardiovascular Surgery of the First Affiliated Hospital, Soochow University, Suzhou 215007, China
5Key Laboratory of Molecular Epigenetics of the Ministry of Education (MOE), Northeast Normal University, Changchun 130024, China
6Hangzhou Rongze Biotechnology, Hangzhou 310000, Zhejiang, China
7Department of Cardiology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China
8Department of Cardiology, Zhongshan Hospital, Fudan University, Shanghai 200032, China
刊名: Molecular Therapy - Nucleic Acids, 2020, Vol.19 , pp.1299-1308
来源数据库: Elsevier Journal
DOI: 10.1016/j.omtn.2020.01.013
关键词: MiR-25Human embryonic stem cellCardiomyocytesProliferation
原始语种摘要: Induction of endogenous cardiomyocyte (CM) proliferation is one of the key strategies for heart regeneration. Increasing evidence points to the potential role of microRNAs (miRNAs) in the regulation of CM proliferation. Here, we used human embryonic stem cell (hESC)-derived CMs (hESC-CMs) as a tool to identify miRNAs that promote CM proliferation. We profiled miRNA expression at an early stage of CM differentiation and identified a list of highly expressed miRNAs. Among these miRNAs, miR-25 was enriched in early-stage hESC-CMs, but its expression decreased over time. Overexpression of miR-25 promoted CM proliferation. RNA sequencing (RNA-seq) analysis revealed that genes related to cell-cycle signal were strongly influenced by miR-25 overexpression. We further showed that miR-25 promoted...
全文获取路径: Elsevier  (合作)

  • expression 表示
  • sequencing 排序
  • demonstrated 探明的储量
  • expressed 榨出的
  • targeting 导向目标
  • further 进一步
  • early 
  • regulation 
  • regeneration 再生