Miniaturized technologies for high-throughput drug screening enzymatic assays and diagnostics – A review
作者: Sarah A.P. PereiraPaul J. DysonM. Lúcia M.F.S. Saraiva
作者单位: 1LAQV, REQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, Rua Jorge Viterbo Ferreira, nº 228, 4050-313, Porto, Portugal
2Institut des Sciences et Ingénierie Chimiques, École Polytechnique Fédérale de Lausanne (EPFL), 1015, Lausanne, Switzerland
刊名: Trends in Analytical Chemistry, 2020, Vol.126
来源数据库: Elsevier Journal
DOI: 10.1016/j.trac.2020.115862
关键词: MiniaturizationDrug screeningEnzyme inhibitionMicroplate formatsMicroarraysMicrofluidicsDiagnostics
英文摘要: Abstract(#br)Drug discovery is a complex, multistep process, in which many challenges need to be overcome at each stage, from the discovery of a biomolecular target to the ensuring of the efficacy and safety of a compound in humans. Today's analytical methods allow tens of thousands of drug candidates to be screened for their ability to inhibit specific enzymes and the miniaturization of these approaches is highly desirable, accelerating the drug discovery process and reducing the associated costs. Herein, it is reviewed the miniaturized techniques currently used to evaluate enzymatic activity and inhibition giving special attention to microplate formats, microarrays, nanoarrays, and microfluidic technologies. It is, also, highlighted some of the characteristics and their abilities for...
全文获取路径: Elsevier  (合作)
影响因子:6.351 (2012)

  • enzymatic 酶催的
  • screening 筛分
  • diagnostics 诊断学
  • throughput 吞吐量