Protein cation exchangers derived by charge reversal from poly(ethylenimine)-Sepharose FF: Comparisons between two derivatization routes
作者: Rui XuXianxiu LiXiaoyan DongYan Sun
作者单位: 1Department of Biochemical Engineering and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China
刊名: Journal of Chromatography A, 2020, Vol.1611
来源数据库: Elsevier Journal
DOI: 10.1016/j.chroma.2019.460586
关键词: Protein chromatographyPoly(ethyleneimine)Charge reductionCharge reversalAdsorption equilibriumUptake kinetics
原始语种摘要: Abstract(#br)It has been known that anion exchangers prepared by grafting poly(ethyleneimine) (PEI) onto Sepharose FF (PEI-Sepharose) at ionic capacities (IC) over 600 mmol/L show both high protein adsorption capacity and uptake kinetics, and charge reversal of PEI-Sepharose by modification with succinic anhydride can produce protein cation exchangers of high capacity and uptake rate. Previously, a Charge Reversal-then-Reduction procedure (route A) was studied for preparation of cation exchangers of different IC values from PEI-Sepharose. In this work, we proposed a new route, that is, Charge Reduction-then-Reversal route (route B), to develop cation exchangers of different IC values from PEI-Sepharose FF with an IC of 700 mmol/L (FF-PEI-L700) as the starting resin. The two kinds of...
全文获取路径: Elsevier  (合作)
影响因子:4.612 (2012)

  • cation 阳离子
  • ethylenimine 氮丙啶
  • reversal 倒转
  • kinetics 动力学
  • adsorption 吸附
  • higher 
  • charge 电荷
  • Adsorption 吸附
  • studied 学习
  • values 价值观