MircroRNA-10b Promotes Human Embryonic Stem Cell-Derived Cardiomyocyte Proliferation via Novel Target Gene LATS1
作者: Yifang XieQiaozi WangNing GaoFujian WuFeng LanFeng ZhangLi JinZheyong HuangJunbo GeHongyan WangYongming Wang
作者单位: 1Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, China
2Obstetrics and Gynecology Hospital, State Key Laboratory of Genetic Engineering at School of Life Sciences, Institute of Reproduction and Development, Fudan University, Shanghai 200011, China
3Key Laboratory of Reproduction Regulation of NPFPC, Collaborative Innovation Center of Genetics and Development, Fudan University, Shanghai 200032, China
4Department of Cardiology, Zhongshan Hospital, Fudan University, Shanghai Institute of Cardiovascular Diseases, Shanghai 200032, China
5State Key Laboratory of Genetic Engineering and School of Life Sciences, Fudan University, Shanghai 200438, China
6Children’s Hospital of Fudan University, Shanghai 201102, China
7Beijing Anzhen Hospital, Beijing Institute of Heart Lung and Blood Vessel Disease, Capital Medical University, Beijing 100029, China
刊名: Molecular Therapy - Nucleic Acids, 2020, Vol.19 , pp.437-445
来源数据库: Elsevier Journal
DOI: 10.1016/j.omtn.2019.11.026
关键词: MiR-10bHuman embryonic stem cellsCardiomyocytesProliferationHippo pathway
原始语种摘要: Adult mammalian cardiomyocytes (CMs) retain a limited proliferative ability, which is insufficient for the repair of CM loss in ischemic cardiac injury. Regulation of the Hippo signaling pathway to promote endogenous CM proliferation has emerged as a promising strategy for heart regeneration. Previous studies have shown that the microRNA cluster miR302–367 negatively regulates the Hippo pathway, promoting CM proliferation. In this study, we identified another microRNA, miR-10b, that regulates the Hippo pathway and promotes cell proliferation in human embryonic stem cell-derived CMs (hESC-CMs). We observed that miR-10b expression was enriched in the early stage of CMs, but its expression was reduced over time. Overexpression of miR-10b promoted CM proliferation, while knockdown of miR-10b...
全文获取路径: Elsevier  (合作)