Development of an ICP-MS/MS approach for absolute quantification and determination of phosphodiester to phosphorothioate ratio in therapeutic oligonucleotides
发展了一种ICP-MS / MS绝对定量和测定治疗性寡核苷酸中磷二酯与硫代磷酸酯比值的方法
作者: Juliusz BiangaMagali PerezDamien MouvetCaroline CajotPhilippe De RaeveArnaud Delobel
作者单位: 1Quality Assistance sa, Technoparc de Thudinie 2, B-6536 Donstiennes, Belgium
刊名: Journal of Pharmaceutical and Biomedical Analysis, 2020, Vol.184
来源数据库: Elsevier Journal
DOI: 10.1016/j.jpba.2020.113179
关键词: Absolute quantificationICP-MS/MSMass spectrometryOligonucleotidePhosphorothioate
原始语种摘要: Abstract(#br)A new analytical method based on ICP-MS/MS is proposed for the characterization of synthetic phosphorothioate oligonucleotides. Absolute quantification of oligonucleotides is challenging, as well as the determination of phosphodiester to phosphorothioate ratio for phosphorothioate oligonucleotides. Both are considered as critical quality attributes and should be determined using robust validated methods. The method we developed was designed to be easy to apply, fast, and robust. It allows simultaneous absolute quantification of an oligonucleotide (based on the quantification of phosphorus), determination of the phosphodiester to phosphorothioate ratio (based on the quantification of phosphorus and sulfur) and optionally determination of sodium (or any other metal) as a...
全文获取路径: Elsevier  (合作)

  • determination 测定
  • phosphodiester 磷酸二酯
  • quantification 量化
  • ratio 
  • absolute 绝对的
  • therapeutic 治疗的
  • phosphorus 
  • potassium 
  • thiophosphate 硫化磷酸盐
  • diethyl 二乙基的