Reconstructing full and partial STR profiles from severely burned human remains using comparative ancient and forensic DNA extraction techniques
作者: M.V. EmeryK. BolhofnerS. WiningearR. OldtM. MontesS. KanthaswamyJ.E. BuikstraL.C. FulginitiA.C. Stone
作者单位: 1School of Human Evolution and Social Change, Arizona State University, United States
2Center for Evolution and Medicine, Arizona State University, United States
3Center for Bioarchaeological Research, Arizona State University, United States
4Maricopa County Office of the Medical Examiner, Phoenix, AZ, United States
5School of Life Sciences, Arizona State University, United States
6School of Mathematical and Natural Sciences, Arizona State University, United States
刊名: Forensic Science International: Genetics, 2020, Vol.46
来源数据库: Elsevier Journal
DOI: 10.1016/j.fsigen.2020.102272
关键词: Burnt human remainsCremainsAncient DNAForensic DNASTR genotypingQuantitative PCR
原始语种摘要: Abstract(#br)Thermal degeneration of the DNA molecule presents a special challenge to medico-legal investigations since low DNA yields, fragmented DNA molecules, and damaged nucleotide bases hinder accurate STR genotyping. As a consequence, fragments of severely burned human remains are often not amenable to standard DNA recovery. However, current ancient DNA (aDNA) extraction methods have proven highly effective at obtaining ultrashort DNA fragments (∼50 bp) from degraded palaeontological and archaeological specimens. In this study, we compare DNA yields and STR results obtained from two established aDNA and forensic DNA extraction protocols by sampling multiple skeletal elements recovered from victims (n = 23) involved in fire-related incidents. DNA yields and STR results suggest an...
全文获取路径: Elsevier  (合作)
影响因子:3.861 (2012)

  • forensic 法庭
  • STR STatus Register
  • extraction 提取
  • remains 残渣
  • DNA Deoxyribonucleic Acid
  • burned 焦化
  • human 人的
  • ancient 古代的
  • severely 严重地
  • nucleotide 核苷酸