SerpinB1 promotes the proliferation of porcine pancreatic stem cells through the STAT3 signaling pathway
作者: Shuanshuan XuDezhe QinHong YangChen HeWenqing LiuNa TianYudong WeiXin HeJinlian HuaSha Peng
作者单位: 1College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering & Technology, Northwest A&F University, Yangling, Shaanxi, 712100, PR China
刊名: Journal of Steroid Biochemistry and Molecular Biology, 2020, Vol.198
来源数据库: Elsevier Journal
DOI: 10.1016/j.jsbmb.2019.105537
关键词: DiabetesPancreatic stem cellsSerpinB1ProliferationInsulin-secreting cells
原始语种摘要: Abstract(#br)Porcine pancreatic stem cells (pPSCs) can be induced to insulin-secreting cells and therefore considered the most promising seeding cells for curing human diabetes in future. However, insufficient pPSCs number is one of the bottleneck problems before its clinical application. SerpinB1 is a serine protease inhibitor in neutrophils and can directly promote the proliferation of β cells. Whether SerpinB1 is involved in pPSC proliferation and differentiation remains unknown. The effects of SerpinB1 on pPSCs proliferation were measured by Cell Counting Kit-8, 5-ethynyl-2′-deoxyuridine, qRT-PCR, western blot, and flow cytometry assays. We found that pPSCs did not efficiently reach the S phase when SerpinB1 expression was knocked down with short hairpin RNA (sh-SerpinB1), the...
全文获取路径: Elsevier  (合作)

  • cells 麻风细胞
  • STAT 静止的
  • insulin 胰岛素
  • pancreatic 胰的
  • proliferation 增生
  • pathway 轨道
  • diabetes 糖尿病
  • expression 表示
  • found 创立
  • remains 残渣