Adsorption mechanism of triterpenoid saponins in reversed-phase liquid chromatography and hydrophilic interaction liquid chromatography: Mogroside V as test substance
三萜皂苷在反相液相色谱和亲水相互作用液相色谱中的吸附机理:以莫格罗苷Ⅴ为供试品
作者: Qing FuLin NiDasen JiangYanxiong KeYu Jin
作者单位: 1Engineering Research Center of Pharmaceutical Process Chemistry, Ministry of Education, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, PR China
刊名: Journal of Chromatography A, 2020, Vol.1620
来源数据库: Elsevier Journal
DOI: 10.1016/j.chroma.2020.461010
关键词: Triterpenoid saponinsMogroside VReversed-phase liquid chromatography (RPLC)Hydrophilic interaction liquid chromatography (HILIC)Adsorption isothermSeparation
原始语种摘要: Abstract(#br)In this paper, adsorption mechanism of triterpenoid saponins in reversed-phase liquid chromatography (RPLC) and hydrophilic interaction liquid chromatography (HILIC) was proposed based on the study of the retention behavior of mogroside V as test substance. The change of peak shape of mogroside V and its influencing factors was first investigated. As the increase of sample loading, a tailing peak of mogroside V was observed in MeOHH 2 O of both two modes. It was the fronting peak in ACNH 2 O of HILIC while there was a transition from fronting peak to tailing peak in ACNH 2 O of RPLC that was largely affected by column temperature and ACN concentration. The adsorption isotherm of mogroside V in ACN...
全文获取路径: Elsevier  (合作)
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影响因子:4.612 (2012)

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关键词翻译
关键词翻译
  • liquid 液体
  • chromatography 色层分离法
  • interaction 相互酌
  • reversed 倒转的
  • phase 相位
  • saponins 皂甙
  • hydrophilic 亲水的
  • isotherm 等温线
  • triterpenoid 三萜系化合物
  • glyph 符号