Pushing the limits of resolving power and analysis time in on-line comprehensive hydrophilic interaction x reversed phase liquid chromatography for the analysis of complex peptide samples
作者: Soraya ChapelFlorent RouvièreSabine Heinisch
作者单位: 1Université de Lyon, Institut des Sciences Analytiques, UMR 5280, CNRS, ENS Lyon, 5 rue de la Doua, Villeurbanne 69100, France
刊名: Journal of Chromatography A, 2020, Vol.1615
来源数据库: Elsevier Journal
DOI: 10.1016/j.chroma.2019.460753
关键词: Two-dimensional liquid chromatographyHILIC x RPLC-HRMSPeptidesBreakthroughSolvent strength mismatch
原始语种摘要: Abstract(#br)In the present work, we have investigated the combination of hydrophilic interaction liquid chromatography (HILIC) and reversed phase liquid chromatography (RPLC) for the separation of peptides in on-line HILIC x RPLC. This combination usually leads to significant solvent strength mismatch, since a weak solvent in HILIC becomes a strong solvent in RPLC. This may result in band broadening, peak distortion, and breakthrough phenomena. Our focus was directed towards the reduction of band broadening and peak distortion. The conditions of the emergence of breakthrough could be investigated with high resolution mass spectrometry (HRMS) detection. The importance of both the injection volume and the difference in composition between injection and elution solvents was highlighted....
全文获取路径: Elsevier  (合作)
影响因子:4.612 (2012)

  • liquid 液体
  • chromatography 色层分离法
  • reversed 倒转的
  • interaction 相互酌
  • phase 相位
  • hydrophilic 亲水的
  • injection 贯入
  • analysis 分析
  • complex 超群
  • breakthrough 突破