Design a highly specific sequence for electrochemical evaluation of meat adulteration in cooked sausages
作者: Maryam MansouriBalal KhalilzadehAboulfazl BarzegariShahram ShoeibiSelim IsildakNasrin BargahiYadollah OmidiSiavoush DastmalchiMohammad-Reza Rashidi
作者单位: 1Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
2Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
3Biosensors and Bioelectronics Research Center, Ardabil University of Medical Sciences, Ardabil, Iran
4Research Center for Pharmaceutical Nanotechnology (RCPN), Tabriz University of Medical Sciences, Tabriz, Iran
5Food and Drug Laboratories Research Center (FDLRC), Iran Food and Drug Administration (IFDA), Ministry of Health and Medical Education (MOH), Tehran, Iran
6Department of Bioengineering, Faculty of Chemical and Metallurgical Engineering, Yildiz Technical University, 34210, Esenler, Istanbul, Turkey
7Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
刊名: Biosensors and Bioelectronics, 2020, Vol.150
来源数据库: Elsevier Journal
DOI: 10.1016/j.bios.2019.111916
关键词: ElectrochemistryAdulterationSausageGenosensorLNAFood analysis
原始语种摘要: Abstract(#br)A specific and unique sequence probe was designed for detection of donkey adulteration in cooked sausages and its species specificity was confirmed bioinformatically in the common software and website (ClustalX and NCBI). Subsequently, a novel species-specific electrochemical DNA probe (locked nucleic acid, LNA) was synthesized and implemented in a construction of DNA-based electrochemical genosensor for sensitive, convenient and selective detection of donkey adulteration. The electrochemical behavior of the fabricated genosensor was studied by linear sweep, square wave, differential pulse voltammetry and electrochemical impedance spectroscopy techniques. Due to inherent optimal hybridization conditions, the lower limit of quantification (LLOQ) was obtained as 148 pM with a...
全文获取路径: Elsevier  (合作)
影响因子:5.437 (2012)