Microfluidic immobilized metal affinity chromatography based on Ti(IV)-decorated silica microspheres for purification of phosphoproteins
作者: Duygu YıldırımÇiğdem KipKhaliunsarnai Tsogtbaatarİlkay KoçerEda ÇelikAli Tuncel
作者单位: 1Hacettepe University, Chemical Engineering Department, Ankara 06800, Turkey
2Hacettepe University, Institute of Science, Division of Bioengineering, 06800 Ankara, Turkey
刊名: Journal of Chromatography B, 2020, Vol.1140
来源数据库: Elsevier Journal
DOI: 10.1016/j.jchromb.2020.122010
关键词: AdsorptionMicrofluidic systemSilicaNanoparticlesMicrospheresEnrichmentImmobilized metal affinity chromatographyCaseinPhosphopeptides
原始语种摘要: Abstract(#br)A silica-based immobilized metal affinity chromatography (IMAC) sorbent with the morphological properties suitable for purification of large phosphorylated biomolecules was synthesized. The sorbent was designed in the form of monodisperse-porous silica microspheres, 5.3 μm in size, having bimodal pore size distribution with a large median pore size (40 nm) and high surface area (163 m 2 /g) decorated with Ti(IV) cations (i.e. Ti(IV)@THSPMP@SiO 2 microspheres). The decoration of silica microspheres with Ti(IV) cations was made by using 3-(trihydroxysilyl)propyl methylphosphonate (THSPMP) as a bifunctiontional linker, by preserving their bimodal pore size distribution. The mesopores provided a large surface area for parking of adsorbed phosphoproteins as large phosphorylated...
全文获取路径: Elsevier  (合作)
影响因子:2.487 (2012)

  • metal 金属
  • IV Increased Value
  • purification 提纯
  • Enrichment 浓缩
  • affinity 亲和力
  • Adsorption 吸附
  • chromatography 色层分离法
  • decorated 具饰纹的
  • silica 硅土
  • system