Highly sensitive detection of salbutamol by ALP-mediated plasmonic ELISA based on controlled growth of AgNPs
作者: Hongwei HeTieqiang SunWentao LiuZehua XuZhenyu HanLingdi ZhaoXinglin WuBaoan NingJialei Bai
作者单位: 1Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment & Food Safety, Tianjin Institute of Environmental and Operational Medicine, No. 1 Dali Road, Tianjin 300050, China
2College of Public Health, Inner Mongolia Medical University, Hohhot 010059, China
刊名: Microchemical Journal, 2020, Vol.156
来源数据库: Elsevier Journal
DOI: 10.1016/j.microc.2020.104804
关键词: SalbutamolPlasmonic ELISASilver nanoparticlesAlkaline phosphataseColorimetric detectionDirect competitive immunoassay
原始语种摘要: Abstract(#br)Salbutamol (SAL), a short-acting β-2 adrenergic receptor agonist, is an undesirable addition to livestock farming and used to improve the growth rates. A direct competitive colorimetric immunoassay for SAL was developed. The alkaline phosphatase (ALP)-mediated controlled growth of the prereduction silver nanoparticles (AgNPs) was used to achieve the highly sensitive, colorimetric detection of SAL through the plasmonic ELISA. Click chemistry reaction was used to synthesize salbutamol biotin label (SAL-Bio) to achieve signal amplification and specific binding with antibody. ALP can efficiently hydrolyze sodium L-ascorbyl-2-phosphate and generate L-ascorbic acid, thereby reducing Ag + and forming AgNPs. The plasmon resonance absorption signals of AgNPs were enhanced along with a...
全文获取路径: Elsevier  (合作)
影响因子:2.879 (2012)

  • ELISA 酶联免疫吸收分析
  • immunoassay 免疫测定法
  • ALP Airborne Line Printer
  • salbutamol 沙丁胺醇
  • controlled 受控
  • detection 探测
  • growth 生长
  • phosphatase 磷酸酶
  • Silver 
  • prereduction 预还原