Molecular cloning of the Bombus terrestris bumblebee venom protein phospholipase A2 and its anti-leukemia effects on K562 cells
作者: Yuling QiuYali ChenHaiyang YuQianxiang ZhouRan WangMeihua JinDexin Kong
作者单位: 1Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmacy, Tianjin Medical University, Tianjin 300070, China
2Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
3Research Center of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China
刊名: Journal of Asia-Pacific Entomology, 2017, Vol.20 (2), pp.699-704
来源数据库: Elsevier Journal
DOI: 10.1016/j.aspen.2017.04.007
关键词: PLA 2Bumblebee venomPurificationAnti-leukemiaApoptosis
英文摘要: Abstract(#br)Bee venom has been used for treating various diseases for a long time. However, the bioactive constituents of bee venom and its mechanisms remain poorly understood. In the present study, phospholipase A 2 ( Bt-PLA 2 ) cDNA was cloned, and a mature form of Bt-PLA 2 was purified from bumblebee venom ( Bombus terrestris ). The differentiation induction and apoptosis induction activities of Bt-PLA 2 on chronic myelogenous leukemia (CML) K562 cells were also evaluated. Bt-PLA 2 cDNA has 540 nucleotides that encode a 180-amino-acid protein. The purified, mature form of Bt-PLA 2 was an 18-kDa protein, and it inhibited K562 cell growth, determined by an IC 50 value of 29.5ng/μl. Moreover, Bt-PLA 2 induced erythroid differentiation of K562 cells in a dose-dependent...
全文获取路径: Elsevier  (合作)
影响因子:0.797 (2012)

  • leukemia 白血病
  • phospholipase 磷脂酶
  • protein 蛋白质
  • venom 毒物
  • bumblebee 扫除音响水雷用发音器
  • cells 麻风细胞
  • cloning 无性繁殖系化
  • PLA phase alternating line
  • effects 海员自身物品